Western Blot:Article Title: Kalirin-7 Mediates Cocaine-Induced AMPA Receptor and Spine Plasticity, Enabling Incentive Sensitization
Article Snippet: .. Immunoblotting was performed as described previously ( Ferrario et al., 2010 ; Boudreau et al., 2012 ) using the following primary antibodies: Kal-spectrin (1:500; catalog #07-122; Millipore), Kal-7 (1:500; catalog #18-202-335512; Genway), PAK1/2/3 (1:1000; catalog #2604; Cell Signaling Technology), p-PAK1(Thr423)/PAK2(Thr402) (1:1000; catalog #2601S; Cell Signaling Technology), phosphotyrosine (1:500; catalog #1400-01; Southern Biotech), phosphothreonine (1:500; catalog #13-9200; Invitrogen), phosphoserine (1:500; catalog #61-8100; Invitrogen), GluA1 (1:1000; catalog #PA1-37776; Thermo Scientific), GluA2 (1:200; catalog #75-002; University of California–Davis/National Institutes of Health NeuroMab Facility), GluA3 (1:1000; catalog #3437S; Cell Signaling Technology), and GluA2/3 (1:2000; catalog #B1506; Millipore). .. After immunoblotting, the immunoreactivity of each band was quantified using Quantity One analysis software (Bio-Rad) and normalized to either total protein in the lane (determined by staining with Ponceau S; catalog #P7170-1L; Sigma-Aldrich) or a loading control (GADPH; catalog #CB1001; Calbiochem).
Virus:Article Title: SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SARS-CoV-2-NP GeneTex Cat# GTX135361; RRID: AB_2887484 Mouse anti-SARS-CoV-2-NP Thermo Fisher Cat# MA1-7403; RRID: AB_1018420 Mouse anti-SARS-CoV-2-SP GeneTex Cat# GTX632604; RRID: AB_2864418 Rabbit anti-ACE2 Abcam Cat# ab15348; RRID: AB_301861 Mouse anti-TMPRSS2 Millipore Cat# MABF2158 Mouse anti-NRP1 Santa Cruz Cat# sc-5307; RRID: AB_2282634 Rabbit anti-NRP1 Abcam Cat# ab81321; RRID: AB_1640739 Rabbit anti-NRP1 Atlas Cat# HPA030278; RRID: AB_10601976 Mouse anti-TFRC Thermo Fisher Cat# 13-6800; RRID: AB_2533029 Rabbit anti-TFRC Atlas Cat# HPA028598; RRID: AB_10601599 Mouse anti-Insulin Cell Signaling Cat# 8138S; RRID: AB_10949314 Mouse anti-Insulin Santa Cruz Cat# sc-8033; RRID: AB_627285 Rabbit anti-glucagon ProteinTech Cat# 15954-1-AP; RRID: AB_2878200 Mouse anti-glucagon Abcam Cat# ab10988; RRID: AB_297642 Mouse-somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse-somatostatin Santa Cruz Cat# sc-74556; RRID: AB_2271061 Mouse anti-CD31 BD Cat# 550389; RRID: AB_2252087 Mouse anti-CD31 Novus Cat# NBP2-47785; RRID: AB_2864381 Rabbit anti- Phospho-JNK1/2 Cell Signaling Cat# 4668T Rabbit anti- Phospho-PAK1/2 Cell Signaling Cat# 2601S; RRID: AB_330220 Bacterial and virus strains SARS-CoV-2 Joe DeRisi Lab, UCSF SARS-CoV-2/human/USA/CA-UCSF- 0001C/2020 Biological samples Human pancreatic islets Integrated Islet Distribution Program (IIDP) Table 2 Chemicals, peptides, and recombinant proteins DMSO Sigma-Aldrich Cat# 276855 EG00229 Sigma-Aldrich Cat# SML1367 DAPI Bio Trend Cat# 40043 Pen/Strep Thermo Fisher Cat# 15140163 Paraformaldehyde AlfaAesar Cat# 433689M Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-121 NP40 Sigma Cat# 11332473001 The unrelated mock peptide: KKHKNQRSRKKHKNQRSR Genscript N/A The NRP1 peptide for blocking assay Abcam Cat# ab189308 The TFRC peptide for blocking assay: DQARSAFSNLFGGEPLSYTRFSLARQ GenScript N/A The SARS-CoV-2 NP peptide for blocking assay: STGSNQNGERSGARSK GenScript N/A BSA Sigma Cat# A6003-25G 1X PBS Corning Cat# 46-013-CM Triton X-100 USB Cat# 22686 OCT compound VWR Cat# 25608-930 (Continued on next page) Cell Metabolism 33, 1565–1576.e1–e5, August 3, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Saponin Sigma-Aldrich Cat# S7900 Fluoromount-G SouthernBiotech Cat# 0100-01 Dako Target Retrieval Solution, pH 9 DAKO Agilent Cat# S236784-2 ProLong Gold Antifade mounting medium with DAPI Thermo Fisher Cat# P36931 Hoechst Thermo Fisher Cat# 33342 GlutaMax life Cat# 35050-079 Critical commercial assays Human insulin ELISA kit Mercodia Cat# 10-1113-01 In Situ Cell Death Detection Kit, TMR red Sigma-Aldrich Cat# 12156792910 TaqMan 2019-nCoV Control Kit v1 Thermo Fisher Cat# A47533 RNAscope Multiplex Fluorescent Reagent Kit v2 Bio-Techne Cat# 323100 TSA Cyanine 3 Akoya Biosciences Cat# NEL744001KT High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Scientific Cat# A32992 Deposited data RNA-seq data for FACS purified human alpha and beta cells (Blodgett et al., 2015) NCBI Gene Expression Omnibus GEO: GSE67543 RNA-seq data for FACS purified human alpha and beta cells (Arda et al., 2016) NCBI Gene Expression Omnibus GEO: GSE57973 RNA-seq data for FACS purified human alpha, beta, and delta cells (Kim et al., 2020) NCBI Gene Expression Omnibus GEO: GSE143889 Phosphoproteomics data of SARS-CoV-2 infected and Spike protein treated human pancreatic islets This paper PRIDE: PXD025629 Oligonucleotides SARS-CoV-2 spike mRNA Bio-Techne Cat# 848561 Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ Zen black Carl Zeiss https://www.zeiss.com/microscopy/us/ products/microscope-software/zen.html ll Clinical and Translational Report
Recombinant:Article Title: SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SARS-CoV-2-NP GeneTex Cat# GTX135361; RRID: AB_2887484 Mouse anti-SARS-CoV-2-NP Thermo Fisher Cat# MA1-7403; RRID: AB_1018420 Mouse anti-SARS-CoV-2-SP GeneTex Cat# GTX632604; RRID: AB_2864418 Rabbit anti-ACE2 Abcam Cat# ab15348; RRID: AB_301861 Mouse anti-TMPRSS2 Millipore Cat# MABF2158 Mouse anti-NRP1 Santa Cruz Cat# sc-5307; RRID: AB_2282634 Rabbit anti-NRP1 Abcam Cat# ab81321; RRID: AB_1640739 Rabbit anti-NRP1 Atlas Cat# HPA030278; RRID: AB_10601976 Mouse anti-TFRC Thermo Fisher Cat# 13-6800; RRID: AB_2533029 Rabbit anti-TFRC Atlas Cat# HPA028598; RRID: AB_10601599 Mouse anti-Insulin Cell Signaling Cat# 8138S; RRID: AB_10949314 Mouse anti-Insulin Santa Cruz Cat# sc-8033; RRID: AB_627285 Rabbit anti-glucagon ProteinTech Cat# 15954-1-AP; RRID: AB_2878200 Mouse anti-glucagon Abcam Cat# ab10988; RRID: AB_297642 Mouse-somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse-somatostatin Santa Cruz Cat# sc-74556; RRID: AB_2271061 Mouse anti-CD31 BD Cat# 550389; RRID: AB_2252087 Mouse anti-CD31 Novus Cat# NBP2-47785; RRID: AB_2864381 Rabbit anti- Phospho-JNK1/2 Cell Signaling Cat# 4668T Rabbit anti- Phospho-PAK1/2 Cell Signaling Cat# 2601S; RRID: AB_330220 Bacterial and virus strains SARS-CoV-2 Joe DeRisi Lab, UCSF SARS-CoV-2/human/USA/CA-UCSF- 0001C/2020 Biological samples Human pancreatic islets Integrated Islet Distribution Program (IIDP) Table 2 Chemicals, peptides, and recombinant proteins DMSO Sigma-Aldrich Cat# 276855 EG00229 Sigma-Aldrich Cat# SML1367 DAPI Bio Trend Cat# 40043 Pen/Strep Thermo Fisher Cat# 15140163 Paraformaldehyde AlfaAesar Cat# 433689M Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-121 NP40 Sigma Cat# 11332473001 The unrelated mock peptide: KKHKNQRSRKKHKNQRSR Genscript N/A The NRP1 peptide for blocking assay Abcam Cat# ab189308 The TFRC peptide for blocking assay: DQARSAFSNLFGGEPLSYTRFSLARQ GenScript N/A The SARS-CoV-2 NP peptide for blocking assay: STGSNQNGERSGARSK GenScript N/A BSA Sigma Cat# A6003-25G 1X PBS Corning Cat# 46-013-CM Triton X-100 USB Cat# 22686 OCT compound VWR Cat# 25608-930 (Continued on next page) Cell Metabolism 33, 1565–1576.e1–e5, August 3, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Saponin Sigma-Aldrich Cat# S7900 Fluoromount-G SouthernBiotech Cat# 0100-01 Dako Target Retrieval Solution, pH 9 DAKO Agilent Cat# S236784-2 ProLong Gold Antifade mounting medium with DAPI Thermo Fisher Cat# P36931 Hoechst Thermo Fisher Cat# 33342 GlutaMax life Cat# 35050-079 Critical commercial assays Human insulin ELISA kit Mercodia Cat# 10-1113-01 In Situ Cell Death Detection Kit, TMR red Sigma-Aldrich Cat# 12156792910 TaqMan 2019-nCoV Control Kit v1 Thermo Fisher Cat# A47533 RNAscope Multiplex Fluorescent Reagent Kit v2 Bio-Techne Cat# 323100 TSA Cyanine 3 Akoya Biosciences Cat# NEL744001KT High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Scientific Cat# A32992 Deposited data RNA-seq data for FACS purified human alpha and beta cells (Blodgett et al., 2015) NCBI Gene Expression Omnibus GEO: GSE67543 RNA-seq data for FACS purified human alpha and beta cells (Arda et al., 2016) NCBI Gene Expression Omnibus GEO: GSE57973 RNA-seq data for FACS purified human alpha, beta, and delta cells (Kim et al., 2020) NCBI Gene Expression Omnibus GEO: GSE143889 Phosphoproteomics data of SARS-CoV-2 infected and Spike protein treated human pancreatic islets This paper PRIDE: PXD025629 Oligonucleotides SARS-CoV-2 spike mRNA Bio-Techne Cat# 848561 Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ Zen black Carl Zeiss https://www.zeiss.com/microscopy/us/ products/microscope-software/zen.html ll Clinical and Translational Report
Blocking Assay:Article Title: SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SARS-CoV-2-NP GeneTex Cat# GTX135361; RRID: AB_2887484 Mouse anti-SARS-CoV-2-NP Thermo Fisher Cat# MA1-7403; RRID: AB_1018420 Mouse anti-SARS-CoV-2-SP GeneTex Cat# GTX632604; RRID: AB_2864418 Rabbit anti-ACE2 Abcam Cat# ab15348; RRID: AB_301861 Mouse anti-TMPRSS2 Millipore Cat# MABF2158 Mouse anti-NRP1 Santa Cruz Cat# sc-5307; RRID: AB_2282634 Rabbit anti-NRP1 Abcam Cat# ab81321; RRID: AB_1640739 Rabbit anti-NRP1 Atlas Cat# HPA030278; RRID: AB_10601976 Mouse anti-TFRC Thermo Fisher Cat# 13-6800; RRID: AB_2533029 Rabbit anti-TFRC Atlas Cat# HPA028598; RRID: AB_10601599 Mouse anti-Insulin Cell Signaling Cat# 8138S; RRID: AB_10949314 Mouse anti-Insulin Santa Cruz Cat# sc-8033; RRID: AB_627285 Rabbit anti-glucagon ProteinTech Cat# 15954-1-AP; RRID: AB_2878200 Mouse anti-glucagon Abcam Cat# ab10988; RRID: AB_297642 Mouse-somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse-somatostatin Santa Cruz Cat# sc-74556; RRID: AB_2271061 Mouse anti-CD31 BD Cat# 550389; RRID: AB_2252087 Mouse anti-CD31 Novus Cat# NBP2-47785; RRID: AB_2864381 Rabbit anti- Phospho-JNK1/2 Cell Signaling Cat# 4668T Rabbit anti- Phospho-PAK1/2 Cell Signaling Cat# 2601S; RRID: AB_330220 Bacterial and virus strains SARS-CoV-2 Joe DeRisi Lab, UCSF SARS-CoV-2/human/USA/CA-UCSF- 0001C/2020 Biological samples Human pancreatic islets Integrated Islet Distribution Program (IIDP) Table 2 Chemicals, peptides, and recombinant proteins DMSO Sigma-Aldrich Cat# 276855 EG00229 Sigma-Aldrich Cat# SML1367 DAPI Bio Trend Cat# 40043 Pen/Strep Thermo Fisher Cat# 15140163 Paraformaldehyde AlfaAesar Cat# 433689M Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-121 NP40 Sigma Cat# 11332473001 The unrelated mock peptide: KKHKNQRSRKKHKNQRSR Genscript N/A The NRP1 peptide for blocking assay Abcam Cat# ab189308 The TFRC peptide for blocking assay: DQARSAFSNLFGGEPLSYTRFSLARQ GenScript N/A The SARS-CoV-2 NP peptide for blocking assay: STGSNQNGERSGARSK GenScript N/A BSA Sigma Cat# A6003-25G 1X PBS Corning Cat# 46-013-CM Triton X-100 USB Cat# 22686 OCT compound VWR Cat# 25608-930 (Continued on next page) Cell Metabolism 33, 1565–1576.e1–e5, August 3, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Saponin Sigma-Aldrich Cat# S7900 Fluoromount-G SouthernBiotech Cat# 0100-01 Dako Target Retrieval Solution, pH 9 DAKO Agilent Cat# S236784-2 ProLong Gold Antifade mounting medium with DAPI Thermo Fisher Cat# P36931 Hoechst Thermo Fisher Cat# 33342 GlutaMax life Cat# 35050-079 Critical commercial assays Human insulin ELISA kit Mercodia Cat# 10-1113-01 In Situ Cell Death Detection Kit, TMR red Sigma-Aldrich Cat# 12156792910 TaqMan 2019-nCoV Control Kit v1 Thermo Fisher Cat# A47533 RNAscope Multiplex Fluorescent Reagent Kit v2 Bio-Techne Cat# 323100 TSA Cyanine 3 Akoya Biosciences Cat# NEL744001KT High-Select Fe-NTA Phosphopeptide Enrichment Kit Thermo Scientific Cat# A32992 Deposited data RNA-seq data for FACS purified human alpha and beta cells (Blodgett et al., 2015) NCBI Gene Expression Omnibus GEO: GSE67543 RNA-seq data for FACS purified human alpha and beta cells (Arda et al., 2016) NCBI Gene Expression Omnibus GEO: GSE57973 RNA-seq data for FACS purified human alpha, beta, and delta cells (Kim et al., 2020) NCBI Gene Expression Omnibus GEO: GSE143889 Phosphoproteomics data of SARS-CoV-2 infected and Spike protein treated human pancreatic islets This paper PRIDE: PXD025629 Oligonucleotides SARS-CoV-2 spike mRNA Bio-Techne Cat# 848561 Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ Zen black Carl Zeiss https://www.zeiss.com/microscopy/us/ products/microscope-software/zen.html ll Clinical and Translational Report
other:Article Title: A FAK-YAP-mTOR signaling axis regulates stem cell-based tissue renewal in mice
Article Snippet: Rabbit polyclonal anti-pPAK1 (Thr423)/pPAK2 (Thr402) , Cell Signaling Technology , Cat# 2601S, RRID:AB_330220.
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